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Pestova and colleagues incubated [32P]β-globin mRNA with 40S ribosomal particles plus the initiation factors and unlabeled competitor RNA combinations indicated at right: blue, no competitor; green, competitor, along with eIF1 and eIF1A, added at time zero; red, competitor, along with eIF1 and eIF1A, added after 5 min of incubation (by which time complex I had formed).
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